Calling Students and Teachers of Microbiology

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As the subject said, I need help with my homework in Microbiology. I couldn't find the answers in the book so I decided to post the other questions that I haven't found an answer. This refers to the culture media being held inside the laboratory. 1) Define the following: a. holding temperature b. pouring temperature c. solidifying temperature (for the bacterial medium) 2) What are the signs of growths in a liquid medium? 3) Reasons why culture tube should be flamed when opened and closed. 4) Reasons why culture tube should be held horizontally. Your help is much appreciated.