please i need your help , anyone know how to use four-parameter logistic method

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please iam broken can anyone tell me how to calculate the result of ELISA DATA using 4PL method using this equation Y = D + ( A - D )/ ( 1 + ( x/ C )^ B ) the raw data for ELISA IS x y 0 0.02 10 0.086 100 0.717 500 2.679 1000 4.284 how i calculate these fators using excel D is the response at infinite analyte concentration, A is the response at zero analyte concentration, x is the analyte concentration, C is the inflection point on the calibration curve (IC50), and B is a slope factor.