Polymerase Chain Reaction (PCR) Optimization

September 11, 2010 10:04am CST
Polymerase Chain Reaction or PCR is a scientific method or technique of amplifying specific DNA sequence and produce millions of copies. Are you familiar in doing PCR? What did you do when you have no amplification or product? (It always happen to me) Can you share some tips to easily detect the culprit why it didn't worked? Thank you very much!
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